Reverse transcription quantitative real-time PCR (RT-qPCR) is the most commonly used method for the quantification of mRNA expression levels. Generally, the starting material for RTqPCR is purified ...

How does UV-Vis spectrophotometry help with the understanding of failed reactions? Genomic analyses, such as next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR), require ...

The scope of real-time quantitative (qPCR) assays for gene-expression analysis in research applications and for sequence-specific nucleic acid detection for diagnostic purposes continues to grow.

Real-time quantitative PCR (qPCR) is a powerful tool to detect and quantify nucleic acids. By incorporating fluorescently labeled probes or fluorescent double-stranded DNA (dsDNA)-binding dyes into ...

To permanently access this Infographic for free, fill out the short form below. There are many different polymerase chain reaction (PCR) workflows, including end-point PCR, qualitative PCR (qPCR), and ...

For ethical and safety reasons it is critical to develop easily implemented assays with high sensitivity and specificity for gene doping surveillance. Two nested quantitative real-time PCR (qPCR) ...

RT-qPCR stands for quantitative reverse transcription polymerase chain reaction, the “RT” not to be mistaken for “real time”. 2 Unlike qPCR that uses a DNA template, the starting material for RT-qPCR ...

Leading PCR and qPCR technology now available for high-throughput applications: With the Biometra TRobot II and qTOWER³ auto, Analytik Jena presents two new thermal cyclers for fully automated PCR and ...

From the start of the COVID-19 pandemic, diagnostic testing has been highlighted as a key part of global measures to contain the spread of SARS-CoV-2. In a media briefing on March 16, 2020, Dr Tedros ...